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adenylate kinase may end up in conversion of ADP into ATP and AMP and thereby confound interpretation from the ADP/ATP specificity experiments. Fixing the conundrum of how some twin-specificity archaeal ligases could use ATP and NAD+ will depend upon capturing crystal structures of those enzymes with Every nucleotide bound.
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(eighteen) have executed a virtual monitor for smaller molecules that would dock to the N-terminal DBD of HuLig1, a domain conserved in all mammalian DNA ligases. They thereby discovered compounds that (i) inhibited nick sealing by human ligases in vitro
S3), however the topology of your LigA clamp is starkly distinct from that on the clamps fashioned by ChVLig and HuLig1. The kissing contacts that close the LigA clamp are sui generis
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